![]() ![]() ![]() The schematic representation of the principle for Capto Core 700 shows a bead with the inactive porous shell and the ligand-activated core. (B) Capto Core 700 - this novel resin combines size exclusion chromatography (SEC) and mixed-mode anion exchange characteristics. This in turn allows sample to be loaded without dilution or a buffer exchange step, resulting in increased productivity. The different possible interaction modes give the resin novel selectivity and and make it salt tolerant. The ligand coupled to the resin surface allows electrostatic interactions as well as hydrophobic interaction, hydrogen bonding, or thiophilic interaction. Examples of multimodal chromatography resins: (A) Capto MMC - this resin is a mixed-mode cation exchanger with the properties of a weak cation exchanger. In other words, you can use MM chromatography to solve challenging purification problems that you cannot solve using AC, IEX, or HIC (Fig 1).įig 1. Mixed mode chromatography resins have a selectivity (the degree of separation of peaks measured at the top of the peak) that differs from that of “traditional” ligands seen in affinity chromatography (AC), ion exchange chromatography (IEX), and hydrophobic interaction chromatography (HIC). In MM chromatography, ligands immobilized to the resin interact with the target protein molecule through multiple types of interactions where size exclusion, ionic, and hydrophobic interactions are the most important (Fig 1). It can be successfully used in research, process development, and bioprocessing to purify biomolecules that are difficult to separate by other chromatography methods. ![]() Mixed mode chromatography (or multimodal chromatography, MM) is a liquid chromatography technique used for the purification of proteins and other biomolecules. ![]()
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